BAC Engineering Core Facility

Reference

BAC Recombineering:

Copeland NG, Jenkins NA, Court DL (2001) “Recombineering: a powerful new tool for mouse functional genomics”. Nat Rev Genet. 2(10): 769-79.

Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG. (2001) “A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA” Genomics. 73(1): 56-65.

Liu P, Jenkins NA, Copeland NG. (2003) “A highly efficient recombineering-based method for generating conditional knockout mutations”Genome Res. 13(3): 476-84.

Yang Y, Sharan SK. (2003) “A simple two-step, 'hit and fix' method to generate subtle mutations in BACs using short denatured PCR fragments.Nucleic Acids Res. 2003 Aug 1;31(15):e80.

BAC Transgene:

Heintz, N. (2001) “BAC to the future: the use of BAC transgenic mice for neuroscience research” Nat Rev Neurosci. Dec; 2(12): 861-7.

Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. (2003) “A gene expression atlas of the central nervous system based on bacterial artificial chromosomes” Nature 425 (6961): 917-25. [GENSAT] www.gensat.org

Conditional mutagenesis:

Lewandoski M. (2001) “Conditional control of gene expression in the mouse” Nat Rev Genet. 2(10): 743-55.